Addgene - pGEX4T moesin 307-577 Plasmid Data

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11639: pGEX4T moesi…
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Plasmid 11639: pGEX4T moesin 307-577

Gene/insert name:
moesin
Insert size:
813
Species:
H. sapiens (human)
Entrez Gene:
MSN ()
Fusion protein or tag:
GST
Terminal:
N terminal on backbone
Mutation:
Carboxy-terminal half of moesin.
Vector backbone:
pGEX4T1
(Search Vector Database)
Backbone manufacturer:
Amersham
Vector type:
Bacterial Expression
Backbone size w/o insert:
4970
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
pGEX5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Vijaya Ramesh
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Interdomain interaction of merlin isoforms and its influence on intermolecular binding to NHE-RF. Gonzalez-Agosti et al (J Biol Chem. 1999 Nov 26. 274(48):34438-42. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11639" in your Materials and Methods section.