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NES-mCherry-cPHx3
(Plasmid #116862)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 116862 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pmCherry-C1
  • Total vector size (bp) 6282
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PLEKHA1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    483
  • Mutation
    amino acids 169-329 (tandem trimer)
  • Entrez Gene
    PLEKHA1 (a.k.a. TAPP1)
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-For
  • 3′ sequencing primer SV40-pArev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2018/09/06/410811 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NES-mCherry-cPHx3 was a gift from Gerry Hammond (Addgene plasmid # 116862 ; http://n2t.net/addgene:116862 ; RRID:Addgene_116862)
  • For your References section:

    A high-avidity biosensor reveals plasma membrane PI(3,4)P2 is predominantly a class I PI3K signaling product. Goulden BD, Pacheco J, Dull A, Zewe JP, Deiters A, Hammond GRV. J Cell Biol. 2019 Mar 4;218(3):1066-1079. doi: 10.1083/jcb.201809026. Epub 2018 Dec 27. 10.1083/jcb.201809026 PubMed 30591513