Addgene - pRS303-LacZ Plasmid Data

Plasmid 1169: pRS303-LacZ

Gene/insert name:
LACZ
Alt name:
6827
Insert size:
3100
Species:
E. coli
Fusion protein or tag:
GAL1:10 promoter
Terminal:
N terminal on backbone
Vector backbone:
pRS303:GAL1-10
(Search Vector Database)
Vector type:
Yeast Expression
Backbone size w/o insert (bp):
5112
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
M13-F20 List of Sequencing Primers
3' sequencing primer:
M13 Rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Susan Lindquist
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Dominant gain-of-function mutations in Hsp104p reveal crucial roles for the middle region. Schirmer et al (Mol Biol Cell 2004 May;15(5):2061-72. Epub 2004 Feb 20. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1169" in your Materials and Methods section.

Price: $65.00

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