V207 pRETRO-Triple-Flag SD - Acceptor
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11709||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size (bp) 6912
Modifications to backboneSplice for 5' Tags
Vector typeMammalian Expression, Retroviral, Cre/Lox ; Acceptor vector
Growth in Bacteria
Copy numberHigh Copy
/ Fusion Protein
- 3xFLAG (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer MSCV (Common Sequencing Primers)
Terms and Licenses
Acceptor vector for the Creator Splice system.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:V207 pRETRO-Triple-Flag SD - Acceptor was a gift from Tony Pawson (Addgene plasmid # 11709)
For your References section:Modification of the Creator recombination system for proteomics applications--improved expression by addition of splice sites. Colwill K, Wells CD, Elder K, Goudreault M, Hersi K, Kulkarni S, Hardy WR, Pawson T, Morin GB. BMC Biotechnol. 2006 . 6():13. 10.1186/1472-6750-6-13 PubMed 16519801