|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||117547||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typePlant Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name2x35S+5'UTR OMEGA (pICH51288) + Sp-eCas9 1.1 (no stop codon) (pEPOR0SP0019 + YFP (pICSL50005) + Nos (pICH41421)
- Promoter 2x35S+5'UTR OMEGA (pICH51288)
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI (destroyed during cloning)
- 3′ cloning site BsaI (destroyed during cloning)
Terms and Licenses
Promoter/5'UTR: 2x35S+5'UTR OMEGA (pICH51288) +CDS: Sp-eCas9 1.1 (no stop codon) (pEPOR0SP0019 +c-terminal tag: YFP (pICSL50005) +3'UTR/terminator: Nos (pICH41421) Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEPOR1CB0011 was a gift from Nicola Patron (Addgene plasmid # 117547 ; http://n2t.net/addgene:117547 ; RRID:Addgene_117547)
For your References section:Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. Raitskin O, Patron N, Schudoma C, West A, Patron N. bioRxiv 422766 10.1101/422766