pMEG_BB3_YL68N_AE
(Plasmid
#117832)
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Purpose(Empty Backbone) GoldenMOCS backbone for 4 TU in Y. lipolytica
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 117832 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepMEG_BB3_YL68N_AE
- Backbone size (bp) 4193
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Vector typeBacterial Expression, Yeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Nourseothricin (clonNat), 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains 34 bp GoldenMOCS-Yali Linker AB
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMEG_BB3_YL68N_AE was a gift from Michael Sauer (Addgene plasmid # 117832 ; http://n2t.net/addgene:117832 ; RRID:Addgene_117832) -
For your References section:
Golden Gate based metabolic engineering strategy for wild-type strains of Yarrowia lipolytica. Egermeier M, Sauer M, Marx H. FEMS Microbiol Lett. 2019 Jan 29. pii: 5304171. doi: 10.1093/femsle/fnz022. 10.1093/femsle/fnz022 PubMed 30698703
