Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||11794||Bacteria in agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Bacterial Resistance(s)No antibiotic necessary
Growth instructionsgrow at 30oC, and store when it is in exponential phase (do not allow it to get too dense).
Copy numberHigh Copy
Alt namepolA recA718
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
For use with pEP PolI (addgene #11722) and pWT PolI (addgene #11721). This strain contains a temp sens mutation in PolI.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:JS200 strain was a gift from Lawrence Loeb (Addgene plasmid # 11794)
For your References section:Targeted gene evolution in Escherichia coli using a highly error-prone DNA polymerase I. Camps M, Naukkarinen J, Johnson BP, Loeb LA. Proc Natl Acad Sci U S A. 2003 Aug 19. 100(17):9727-32. 10.1073/pnas.1333928100 PubMed 12909725