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JS200 strain
(Bacterial strain #11794)

No maps are available for this item.

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Bacterial Strain 11794 Bacteria in agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    No antibiotic necessary
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    grow at 30oC, and store when it is in exponential phase (do not allow it to get too dense).
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    JS200
  • Alt name
    polA recA718

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

For use with pEP PolI (addgene #11722) and pWT PolI (addgene #11721). This strain contains a temp sens mutation in PolI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    JS200 strain was a gift from Lawrence Loeb (Addgene plasmid # 11794)
  • For your References section:

    Targeted gene evolution in Escherichia coli using a highly error-prone DNA polymerase I. Camps M, Naukkarinen J, Johnson BP, Loeb LA. Proc Natl Acad Sci U S A. 2003 Aug 19. 100(17):9727-32. 10.1073/pnas.1333928100 PubMed 12909725