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Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteria
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 118497 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFru97
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Vector typeBacterial Expression
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Selectable markersGentamicin ; Chloramphenicol/
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)E. coli S17-1 lambda pir
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemScarlet-I
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bypTriEx-RhoA-wt_mScarlet-I_SGFP2
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/early/2018/09/07/402172 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMRE145 was a gift from Mitja Remus-Emsermann (Addgene plasmid # 118497 ; http://n2t.net/addgene:118497 ; RRID:Addgene_118497) -
For your References section:
Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal Insertion Toolbox for Fluorescent Protein Expression in Bacteria. Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, Dobson RCJ, Remus DM, Remus-Emsermann MNP. Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. eCollection 2018. 10.3389/fmicb.2018.03052 PubMed 30631309