pMRE-Tn7-161
(Plasmid
#118573)
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PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 118573 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGRG36
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Vector typeBacterial Expression ; miniTn7 delivery vector
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Selectable markersChloramphenicol/ Tetracycline
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)E. coli S17-1 lambda pir
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namemTurquoise2
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypLifeAct-mTurquoise2
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/early/2018/09/07/402172 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMRE-Tn7-161 was a gift from Mitja Remus-Emsermann (Addgene plasmid # 118573 ; http://n2t.net/addgene:118573 ; RRID:Addgene_118573) -
For your References section:
Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal Insertion Toolbox for Fluorescent Protein Expression in Bacteria. Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, Dobson RCJ, Remus DM, Remus-Emsermann MNP. Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. eCollection 2018. 10.3389/fmicb.2018.03052 PubMed 30631309