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PurposeGateway cloning vector for the cell cycle indicator PIP-FUCCI
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 118621 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDONR221
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Backbone manufacturerThermoFisher Scientific
- Backbone size w/o insert (bp) 2500
- Total vector size (bp) 4560
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Modifications to backbonenone
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Vector typeGateway cloning
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePIP-FUCCI
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SpeciesSynthetic
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Insert Size (bp)1992
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Tags
/ Fusion Proteins
- mVenus (C terminal on insert)
- mCherry (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR-PIP-FUCCI was a gift from Jean Cook (Addgene plasmid # 118621 ; http://n2t.net/addgene:118621 ; RRID:Addgene_118621) -
For your References section:
Accurate delineation of cell cycle phase transitions in living cells with PIP-FUCCI. Grant GD, Kedziora KM, Limas JC, Cook JG, Purvis JE. Cell Cycle. 2018;17(21-22):2496-2516. doi: 10.1080/15384101.2018.1547001. 10.1080/15384101.2018.1547001 PubMed 30421640
Map uploaded by the depositor.
