Purpose(Empty Backbone) To clone sgRNA for activation dCas9
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||118650||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 10425
- Promoter U6
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer U6 primer (Common Sequencing Primers)
Digested with BsmBI
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Lenti_sgRNA_MS2_neo was a gift from Christopher Vakoc (Addgene plasmid # 118650 ; http://n2t.net/addgene:118650 ; RRID:Addgene_118650)
For your References section:A Transcription Factor Addiction in Leukemia Imposed by the MLL Promoter Sequence. Lu B, Klingbeil O, Tarumoto Y, Somerville TDD, Huang YH, Wei Y, Wai DC, Low JKK, Milazzo JP, Wu XS, Cao Z, Yan X, Demerdash OE, Huang G, Mackay JP, Kinney JB, Shi J, Vakoc CR. Cancer Cell. 2018 Dec 10;34(6):970-981.e8. doi: 10.1016/j.ccell.2018.10.015. Epub 2018 Nov 29. 10.1016/j.ccell.2018.10.015 PubMed 30503706