Addgene - C-Ag15 Plasmid Data

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11868: C-Ag15
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Plasmid 11868: C-Ag15

Gene/insert name:
Agrn
Alt name:
Agrin
Insert size:
417
Species:
M. musculus (mouse)
GenBank ID:
NM_021604
Entrez Gene:
Agrn (Agrin)
Fusion protein or tag:
myc
Terminal:
C terminal on backbone
Fusion protein or tag:
His
Terminal:
C terminal on backbone
Vector backbone:
pTrcHis2
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Bacterial Expression
Backbone size w/o insert:
4406
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
AGAGGTATATATTAATGTATCG List of Sequencing Primers
3' sequencing primer:
N/A
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Martin Smith
Terms and Licenses:
MTA

Comments:

Hilgenberg, L. G., Su, H., Gu, H., O'Dowd D, K. & Smith, M. A. (2006) alpha3Na+/K+-ATPase Is a neuronal receptor for agrin. Cell 125, 359-69.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The COOH-terminal domain of agrin signals via a synaptic receptor in central nervous system neurons. Hoover et al (J Cell Biol. 2003 Jun 9. 161(5):923-32. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11868" in your Materials and Methods section.