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pFUGW-GtACR1_C102A-EYFP
(Plasmid #119196)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 119196 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Anion channelrhodopsin GtACR1 step-function mutant C102A
  • Species
    Guillardia theta
  • Insert Size (bp)
    885
  • Mutation
    changed cysteine 102 to alanine
  • GenBank ID
    KP171708 KP171708
  • Promoter Ubiquitin
  • Tag / Fusion Protein
    • EYFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer ATGCGCTCGGGGTTGGCGAGTGTG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFUGW-GtACR1_C102A-EYFP was a gift from John Spudich (Addgene plasmid # 119196 ; http://n2t.net/addgene:119196 ; RRID:Addgene_119196)
  • For your References section:

    Extending the Time Domain of Neuronal Silencing with Cryptophyte Anion Channelrhodopsins. Govorunova EG, Sineshchekov OA, Hemmati R, Janz R, Morelle O, Melkonian M, Wong GK, Spudich JL. eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-0174-18. doi: 10.1523/ENEURO.0174-18.2018. eCollection 2018 May-Jun. eN-MNT-0174-18 [pii] PubMed 30027111