pK218TK620T
(Plasmid #1192)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pRS313
  • Backbone manufacturer
    Hieter
  • Backbone size w/o insert (bp) 4967
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

  • Depositor Sequences
    None.
  • Addgene Sequences

    None. Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Hsp104
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    3314
  • Mutation
    changed lysine 218 to threonine and lysine 620 to threonine
  • GenBank ID
    NC_001144
  • Entrez Gene
    HSP104 (a.k.a. YLL026W)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pK218TK620T was a gift from Susan Lindquist (Addgene plasmid # 1192)
  • For your References section:

    Support for the prion hypothesis for inheritance of a phenotypic trait in yeast. Patino MM, Liu JJ, Glover JR, Lindquist S. Science 1996 Aug 2;273(5275):622-6. 10.1126/science.273.5275.622 PubMed 8662547