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Plasmid 1192: pK218TK620T
  • Hsp104

  • 3314

  • S. cerevisiae (budding yeast)

  • NC_001144

  • HSP104 (YLL026W)

  • changed lysine 218 to threonine and lysine 620 to threonine

  • pRS313
    (Search Vector Database)

  • Hieter

  • Yeast Expression

  • 4967

  • XhoI

  • No

  • SacI

  • No

  • n/a List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • HIS3

  • Susan Lindquist

  • UBMTA

This plasmid has not been sequence verified. Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Support for the prion hypothesis for inheritance of a phenotypic trait in yeast. Patino et al (Science 1996 Aug 2;273(5275):622-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1192" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only
This is commonly requested with