Addgene - pK218TK620T Plasmid Data

Plasmid 1192: pK218TK620T

Gene/insert name:
Hsp104
Insert size:
3314
Species:
S. cerevisiae (budding yeast)
GenBank ID:
NC_001144
Entrez Gene:
HSP104 (YLL026W)
Mutation:
changed lysine 218 to threonine and lysine 620 to threonine
Vector backbone:
pRS313
(Search Vector Database)
Backbone manufacturer:
Hieter
Vector type:
Yeast Expression
Backbone size w/o insert (bp):
4967
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
SacI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
HIS3
Principal Investigator:
Susan Lindquist
Terms and Licenses:
MTA

This plasmid has not been sequence verified. If you would like Addgene to sequence a portion of this plasmid before you request it, please contact us.

Article: Support for the prion hypothesis for inheritance of a phenotypic trait in yeast. Patino et al (Science 1996 Aug 2;273(5275):622-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1192" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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