Addgene - GFP-Ub KO.G76V Plasmid Data

Plasmid 11932: GFP-Ub KO.G76V

Gene/insert name:
Ubiquitin KO.G76V
Alt name:
Ubiquitin
Alt name:
ubiquitin
Alt name:
Ub
Insert size:
245
Species:
H. sapiens (human)
Fusion protein or tag:
GFP
Terminal:
N terminal on backbone
Mutation:
Conjugation deficient mutant. All 7 lysines of ubiquitin mutated to arginines. Glycine 76 mutated to valine.
Vector backbone:
EGFP-C1
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4731
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
KpnI
Site destroyed during cloning:
No
5' sequencing primer:
EGFP-C (CATGGTCCTGCTGGAGTTCGTG) List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (3)
Principal Investigator:
Nico Dantuma
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A dynamic ubiquitin equilibrium couples proteasomal activity to chromatin remodeling. Dantuma et al (J Cell Biol. 2006 Apr 10. 173(1):19-26. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11932" in your Materials and Methods section.

Price: $65.00

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