Addgene - YFP-CL1 Plasmid Data

Plasmid 11950: YFP-CL1

Gene/insert name:
YFP-CL1
Alt name:
Ubiquitin
Alt name:
Ub
Insert size:
826
Vector backbone:
EGFP-C1
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
3932
Cloning site 5':
NheI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
EGFP-C List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Principal Investigator:
Nico Dantuma
Terms and Licenses:
MTA

Comments:

The YFP-CL1 reporter was generated by inserting an oligodimer encoding the 16 amino acid CL1 degradation signal (ACKNWFSSLSHFVIHL) in enhanced GFP-C1 (BD Biosciences Clontech) using the EcoRI and BamH1 restriction sites, then the enhanced GFP open-reading frame was replaced with YFP using the Nhe1 and SspB1 restriction sites.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Endoplasmic reticulum stress compromises the ubiquitin-proteasome system. Menendez-Benito et al (Hum Mol Genet. 2005 Oct 1. 14(19):2787-99. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11950" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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