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pCT5-bac2.0
(Plasmid #119872)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 119872 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRBBm34
  • Backbone manufacturer
    Biedendieck et al., 2007
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For Bacillus: Tetracycline and 30 °C or 37 °C
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    CymR
  • Species
    Pseudomonas putida F1
  • Insert Size (bp)
    624
  • Promoter PxylR promoter

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    sfGFP
  • Species
    Synthetic; superfolding GFP
  • Insert Size (bp)
    717
  • Promoter Pveg promoter with CuO sequence

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCT5-bac2.0 was a gift from Claudia Schmidt-dannert (Addgene plasmid # 119872 ; http://n2t.net/addgene:119872 ; RRID:Addgene_119872)
  • For your References section:

    Development of a synthetic cumate-inducible gene expression system for Bacillus. Seo SO, Schmidt-Dannert C. Appl Microbiol Biotechnol. 2018 Nov 3. pii: 10.1007/s00253-018-9485-4. doi: 10.1007/s00253-018-9485-4. 10.1007/s00253-018-9485-4 PubMed 30392122