pDEST-Hemmar-DSCP-ftz-FLP(D)
(Plasmid #119894)

• Purpose: FLP(D) with a Drosophila Synthetic Core Promoter (DSCP) and ftz intron
• Depositing Lab: Rita Sousa-Nunes
• Publication: Chai et al Elife. 2018 Nov 27;7. pii: 38393. doi: 10.7554/eLife.38393.

Backbone

• Vector backbone: pDEST-HemmarG
• Backbone manufacturer: Addgene
• Backbone size w/o insert (bp): 14231
• Total vector size (bp): 13264
• Vector type: Drosophila expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: FLP(D)
• Species: D. melanogaster (fly)
• Insert Size (bp): 1268
• Mutation: a mutated form of FLP, which at position 5 contains an aspartic acid instead of glycine residue

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: gac ctg cag gCT CGA GAA TCA AAA TGC C
• 3′ sequencing primer: act ggc tta gtt aat taa ttc tag atc tag att aAA TAC GGC GAT TGA TGT AG

Gene/Insert 2

• Gene/Insert name: DSCP-ftz
• Species: D. melanogaster (fly)
• Insert Size (bp): 146
• Entrez Gene: ftz (a.k.a. Dmel_CG2047, BG:DS07876.1, CG2047, Dm-Ftz, Dm-ftz, DmFtz, Dmel\CG2047, Dmftz, FTZ, Ftz, Ual, l(3)84Ag)

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: tcg ttc agc tGA ATT GGC CGC GTT TAA AC
• 3′ sequencing primer: gat tct cga gCC TGC AGG TCT TTG CAA TC

Resource Information

• Supplemental Documents:
  • pDEST-Hemmar-DSCP-ftz-FLP(D)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2018/06/07/341560 for BioRxiv preprint