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LAMP1-SBP-GFP
(Plasmid #120172)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 120172 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4692
  • Total vector size (bp) 6074
  • Modifications to backbone
    Contains mutation A206K for producing monomeric eGFP
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lysosomal-associated membrane protein 1
  • Alt name
    LAMP1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1382
  • Entrez Gene
    Lamp1 (a.k.a. LGP120)
  • Promoter CMV
  • Tags / Fusion Proteins
    • SBP (C terminal on insert)
    • eGFP (C terminal on backbone)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LAMP1-SBP-GFP was a gift from Juan Bonifacino (Addgene plasmid # 120172 ; http://n2t.net/addgene:120172 ; RRID:Addgene_120172)
  • For your References section:

    Reversible association with motor proteins (RAMP): A streptavidin-based method to manipulate organelle positioning. Guardia CM, De Pace R, Sen A, Saric A, Jarnik M, Kolin DA, Kunwar A, Bonifacino JS. PLoS Biol. 2019 May 17;17(5):e3000279. doi: 10.1371/journal.pbio.3000279. eCollection 2019 May. 10.1371/journal.pbio.3000279 PubMed 31100061