Addgene - pAG177 HIPK1 3'UTR mut Plasmid Data

Plasmid 12035: pAG177 HIPK1 3'UTR mut

Gene/insert name:
N10 3'UTR mut
Alt name:
HIPK1
Insert size:
680
Species:
H. sapiens (human)
GenBank ID:
NM_181358
Entrez Gene:
HIPK1 (Myak, Nbak2, KIAA0630, MGC26642, MGC33446, MGC33548)
Mutation:
Mutations in microRNA recognition sites (seed matches): 1860-1865 and 2182-2187.
Vector backbone:
pIS2
(Search Vector Database)
Backbone manufacturer:
pIS2 derived from pRL-SV40 (Promega)
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
3700
Cloning site 5':
SacI
Site destroyed during cloning:
No
Cloning site 3':
SpeI
Site destroyed during cloning:
No
5' sequencing primer:
EBV rev primer (GTGGTTTGTCCAAACTCATC) List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
David Bartel
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

HIPK1 3'UTR mutant in renilla luciferase reporter plasmid.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The widespread impact of mammalian MicroRNAs on mRNA repression and evolution. Farh et al (Science. 2005 Dec 16. 310(5755):1817-21. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12035" in your Materials and Methods section.