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Lyn-R-GECO1
(Plasmid #120410)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 120410 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Customized Vector
  • Backbone size w/o insert (bp) 3930
  • Total vector size (bp) 5266
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lyn-R-GECO1
  • Insert Size (bp)
    1336
  • GenBank ID
    JN258411
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40-pAR
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lyn-R-GECO1 was a gift from Won Do Heo (Addgene plasmid # 120410 ; http://n2t.net/addgene:120410 ; RRID:Addgene_120410)
  • For your References section:

    Optogenetic toolkit reveals the role of Ca2+ sparklets in coordinated cell migration. Kim JM, Lee M, Kim N, Heo WD. Proc Natl Acad Sci U S A. 2016 May 24;113(21):5952-7. doi: 10.1073/pnas.1518412113. Epub 2016 May 17. 10.1073/pnas.1518412113 PubMed 27190091