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pcDNA-DDX11-Flag-KAK
(Plasmid #120729)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 120729 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA 3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Total vector size (bp) 8230
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DDX11 KAK mutant
  • Alt name
    Chlr1 KAK
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2784
  • Mutation
    Substitution of E201, Y202 and E203 with K, A and K
  • Entrez Gene
    DDX11 (a.k.a. CHL1, CHLR1, KRG2, WABS)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3x FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Mutations were created by a PCR based method.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-DDX11-Flag-KAK was a gift from Francesca Pisani (Addgene plasmid # 120729 ; http://n2t.net/addgene:120729 ; RRID:Addgene_120729)
  • For your References section:

    Interaction of the Warsaw breakage syndrome DNA helicase DDX11 with the replication fork-protection factor Timeless promotes sister chromatid cohesion. Cortone G, Zheng G, Pensieri P, Chiappetta V, Tate R, Malacaria E, Pichierri P, Yu H, Pisani FM. PLoS Genet. 2018 Oct 10;14(10):e1007622. doi: 10.1371/journal.pgen.1007622. eCollection 2018 Oct. PGENETICS-D-18-00612 [pii] PubMed 30303954