Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pIA33
(Plasmid #120803)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 120803 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAP114
  • Backbone size w/o insert (bp) 6400
  • Total vector size (bp) 12940
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Low copy in C. difficile following conjugation and selected with thiamphenicol
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    deactivated nuclease dCas9, codon-optimized for C. difficile
  • Alt name
    dCas9-opt
  • Species
    S. pyogenes
  • Insert Size (bp)
    4107
  • Mutation
    Codon optimized for C. difficile; H840A and D10A to deactivate nuclease
  • Promoter Pxyl

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    single guide RNA targeting red fluorescent protein
  • Alt name
    sgRNA-rfp
  • Species
    Synthetic; S. pyogenes
  • Insert Size (bp)
    140
  • Mutation
    Base-pairing region targets red fluorescent protein (rfp)
  • Promoter Pgdh

Cloning Information for Gene/Insert 2

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIA33 was a gift from Craig Ellermeier & David S. Weiss (Addgene plasmid # 120803 ; http://n2t.net/addgene:120803 ; RRID:Addgene_120803)
  • For your References section:

    A xylose-inducible expression system and a CRISPRi-plasmid for targeted knock-down of gene expression in Clostridioides difficile. Muh U, Pannullo AG, Weiss DS, Ellermeier CD. J Bacteriol. 2019 Feb 11. pii: JB.00711-18. doi: 10.1128/JB.00711-18. 10.1128/JB.00711-18 PubMed 30745377