PurposeMoClo golden gate assembly DE part for gN2 gRNA (guide RNA for S. pyogenes Cas9; sequence designed with NUPACK for stable tracrRNA structure and open targeting region).
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||121040||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2100
Vector typeSynthetic Biology
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namegN2 Cas9 gRNA UC part
Insert Size (bp)110
- Cloning method Gibson Cloning
- 5′ sequencing primer AAATAGGCGTATCACGAGGCA
- 3′ sequencing primer TTACCGCCTTTGAGTGAGCTG (Common Sequencing Primers)
Please see Supplemental Documents for annotated Genbank file.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:UC20m was a gift from Richard Murray (Addgene plasmid # 121040 ; http://n2t.net/addgene:121040 ; RRID:Addgene_121040)