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Plasmid 12105: LV-Cre-SD
  • nlsCre

  • nls Cre

  • LV-Cre

  • cre

  • 1867

  • Enterobacteriophage P1

  • cre (P1_gp003)

  • loxP site introduced downstream of nlsCre

  • n/a
    (Search Vector Database)

  • Mammalian Expression, Lentiviral, Cre/Lox

  • 7766

  • EcoRI

  • No

  • KpnI

  • No

  • n/a List of Sequencing Primers

  • Ampicillin

  • Stbl3

  • 37

  • Low Copy

  • none

  • The cre is from pMC-Cre (Gu et al. 1993 Cell 73, 1155-1164

  • View sequences (2)
  • View map

  • Notes from Addgene (1)

  • Inder Verma

  • UBMTA

Comments: 

LV-Cre-SD minimizes the cytotoxic effects of Cre by causing Cre to be recombined out after transient expression. See article for details.

Note that this plasmid is VERY low copy. Grow in a high volume to acquire usable amounts.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Delivery of the Cre recombinase by a self-deleting lentiviral vector: efficient gene targeting in vivo. Pfeifer et al (Proc Natl Acad Sci U S A. 2001 Sep 25. 98(20):11450-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12105" in your Materials and Methods section.

Discontinued

Addgene no longer distributes this plasmid. Contact help@addgene.org for more information