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LV-Cre-SD
(Plasmid #12105)

Backbone

  • Vector backbone
    n/a
  • Backbone size w/o insert (bp) 7766
  • Vector type
    Mammalian Expression, Lentiviral, Cre/Lox
  • Selectable markers
    none

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Low Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    nlsCre
  • Alt name
    nls Cre
  • Alt name
    LV-Cre
  • Alt name
    cre
  • Species
    Enterobacteriophage P1
  • Insert Size (bp)
    1867
  • Mutation
    loxP site introduced downstream of nlsCre
  • Entrez Gene
    cre (a.k.a. P1_gp003)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    The cre is from pMC-Cre (Gu et al. 1993 Cell 73, 1155-1164
  • Terms and Licenses

Depositor Comments

LV-Cre-SD minimizes the cytotoxic effects of Cre by causing Cre to be recombined out after transient expression. See article for details.

Note that this plasmid is VERY low copy. Grow in a high volume to acquire usable amounts.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LV-Cre-SD was a gift from Inder Verma (Addgene plasmid # 12105)
  • For your References section:

    Delivery of the Cre recombinase by a self-deleting lentiviral vector: efficient gene targeting in vivo. Pfeifer A, Brandon EP, Kootstra N, Gage FH, Verma IM. Proc Natl Acad Sci U S A. 2001 Sep 25. 98(20):11450-5. 10.1073/pnas.201415498 PubMed 11553794