Addgene - LV-Cre-SD Plasmid Data

Plasmid 12105: LV-Cre-SD

Gene/insert name:
nlsCre
Alt name:
nls Cre
Alt name:
LV-Cre
Alt name:
cre
Insert size:
1867
Species:
Enterobacteriophage P1
Entrez Gene:
cre (P1_gp003)
Mutation:
loxP site introduced downstream of nlsCre
Vector backbone:
n/a
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, Cre/Lox
Backbone size w/o insert (bp):
7766
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
KpnI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
Low Copy
Selectable markers, other:
none
Person or lab that originally
cloned the gene/insert:
The cre is from pMC-Cre (Gu et al. 1993 Cell 73, 1155-1164
Sequence: View sequences (2)
Map:
View map
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Inder Verma
Terms and Licenses:
MTA

Comments:

LV-Cre-SD minimizes the cytotoxic effects of Cre by causing Cre to be recombined out after transient expression. See article for details.

Note that this plasmid is VERY low copy. Grow in a high volume to acquire usable amounts.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12105" in your Materials and Methods section.