pUC57-Kan_FR13_00066391
(Plasmid
#121301)
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PurposeLevel 0 Golden-Gate compatible vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 121301 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57-Kan
- Backbone size w/o insert (bp) 2626
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Vector typeGolden Gate
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Selectable markerslacZ
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFR13_00066391
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MutationMagnaporthe oryzae
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (unknown if destroyed)
- 3′ cloning site EcoRV (unknown if destroyed)
- 5′ sequencing primer M13Fw
- 3′ sequencing primer M13Rv (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC57-Kan_FR13_00066391 was a gift from Sophien Kamoun (Addgene plasmid # 121301 ; http://n2t.net/addgene:121301 ; RRID:Addgene_121301) -
For your References section:
A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence and Structural Similarities Across Host-Specific Lineages. Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S. Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22. 10.1094/MPMI-03-20-0052-A PubMed 32460610