Addgene - pBK2 Plasmid Data

Plasmid 1214: pBK2

Gene/insert name:
Hsp82::LEU2
Alt name:
hsp90
Alt name:
heat shock
Alt name:
hsp82
Insert size:
2800
Species:
S. cerevisiae (budding yeast)
GenBank ID:
NC_001148
Entrez Gene:
HSP82 (YPL240C, HSP90)
Mutation:
hsp82::LEU2 select for on LEU2 excise fragment using HindIII and MluI
Vector backbone:
Bluescribe
(Search Vector Database)
Backbone manufacturer:
Stratagene
Vector type:
Yeast Expression ; integration
Backbone size w/o insert (bp):
3200
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Unknown
Growth temperature (℃):
37
Growth instructions:
BSJ72
High or low copy:
Unknown
Principal Investigator:
Susan Lindquist
Terms and Licenses:
MTA

This plasmid has not been sequence verified. If you would like Addgene to sequence a portion of this plasmid before you request it, please contact us.

Article: Mutational analysis of Hsp90 function: interactions with a steroid receptor and a protein kinase. Nathan et al (Mol Cell Biol 1995 Jul;15(7):3917-25. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1214" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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