JE301: pMVP (L5-L4) Tir1-P2A-AID
PurposepMVP L5-L4 entry plasmid, contains Tir1-P2A-AID for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Tir1 linked by P2A to AID domain fused to gene of interest.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||121722||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepDONR221 P5-P4
- Backbone size w/o insert (bp) 2554
Vector typeSynthetic Biology ; pMVP Gateway Entry Plasmid
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameosTir1-P2A-AID (open)
SpeciesSynthetic; O. Sativa
Insert Size (bp)2451
/ Fusion Protein
- 9x myc (C-term on osTIR1) (C terminal on insert)
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
osTir1/9xmyc sequence PCR'd from Addgene #47328. Minimal auxin-inducible degron (AID) sequence derived from PMID: 27052166.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:JE301: pMVP (L5-L4) Tir1-P2A-AID was a gift from Christopher Newgard (Addgene plasmid # 121722 ; http://n2t.net/addgene:121722 ; RRID:Addgene_121722)
For your References section:Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing. Haldeman JM, Conway AE, Arlotto ME, Slentz DH, Muoio DM, Becker TC, Newgard CB. Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286. 10.1093/nar/gky1286 PubMed 30590691