KK601: pMVP (L3-L2) ecDHFR DD + polyA
PurposepMVP L3-L2 entry plasmid, contains ecDHFR DD + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term ecDHFR degron domain (stabilized by TMP) to gene of interest.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||121790||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepDONR221 P3-P2
- Backbone size w/o insert (bp) 2555
Vector typeSynthetic Biology ; pMVP Gateway Entry Plasmid
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameecDHFR Degron Domain + polyA
Insert Size (bp)735
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
ecDHFR degron domain is stabilized by the ligand TMP
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:KK601: pMVP (L3-L2) ecDHFR DD + polyA was a gift from Christopher Newgard (Addgene plasmid # 121790 ; http://n2t.net/addgene:121790 ; RRID:Addgene_121790)
For your References section:Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing. Haldeman JM, Conway AE, Arlotto ME, Slentz DH, Muoio DM, Becker TC, Newgard CB. Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286. 10.1093/nar/gky1286 PubMed 30590691