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pcDNA3 MEKK1 WT
(Plasmid #12181)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12181 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1/HisB
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MEKK1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    5200
  • Mutation
    V1467L
  • GenBank ID
    AF117340
  • Entrez Gene
    Map3k1 (a.k.a. MAPKKK1, MEKK1, Mekk)
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • Xpress (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site unknown (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing results identified a V1467L mutation in the MEKK1 sequence when compared to GenBank ID AF117340.1. The depositing laboratory states that this plasmid functions exactly as intended and the mutation may represent a valid polymorphism.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 MEKK1 WT was a gift from Gary Johnson (Addgene plasmid # 12181 ; http://n2t.net/addgene:12181 ; RRID:Addgene_12181)
  • For your References section:

    MEK kinase 1, a substrate for DEVD-directed caspases, is involved in genotoxin-induced apoptosis. Widmann C, Gerwins P, Johnson NL, Jarpe MB, Johnson GL. Mol Cell Biol. 1998 Apr . 18(4):2416-29. PubMed 9528810