JI702: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; RIP
Purpose(Empty Backbone) pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||121820||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepDONR221 P5-P4
- Backbone size (bp) 2554
Vector typeSynthetic Biology ; pMAGIC Gateway Entry Plasmid
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namehU6::SaCas9 gRNA scaffold + RIP
Insert Size (bp)835
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Annealed oligonucleotides containing the protospacer motif can be ligated into BsaI digested plasmid. SaCas9 gRNA scaffold derived from PMID: 26721686
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:JI702: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; RIP was a gift from Christopher Newgard (Addgene plasmid # 121820 ; http://n2t.net/addgene:121820 ; RRID:Addgene_121820)
For your References section:Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing. Haldeman JM, Conway AE, Arlotto ME, Slentz DH, Muoio DM, Becker TC, Newgard CB. Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286. 10.1093/nar/gky1286 PubMed 30590691