KN901: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-KRAB
PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the KRAB transcriptional repressor for 3 or 4-component MultiSite Gateway Pro assembly.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||121830||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepDONR221 P4r-P3r
- Backbone size w/o insert (bp) 2664
Vector typeSynthetic Biology ; pMAGIC Gateway Entry Plasmid
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namex-dCas9(3.7)/KRAB (open)
Insert Size (bp)4449
/ Fusion Proteins
- NLS (N terminal on insert)
- NLS (C terminal on insert)
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
x-dCas9(3.7) PCR'd from Addgene #108383, KRAB derived from Addgene #60954
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:KN901: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-KRAB was a gift from Christopher Newgard (Addgene plasmid # 121830 ; http://n2t.net/addgene:121830 ; RRID:Addgene_121830)
For your References section:Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing. Haldeman JM, Conway AE, Arlotto ME, Slentz DH, Muoio DM, Becker TC, Newgard CB. Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286. 10.1093/nar/gky1286 PubMed 30590691