pHBBAD113
(Plasmid
#121912)
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PurposeCharge-Introduced NpuDnaBC39 intein
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 121912 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBAD
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 4798
- Total vector size (bp) 4918
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Modifications to backboneGB1-His6 tag
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCI-NpuDnaBC39 intein
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SpeciesNostoc punctiforme
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Insert Size (bp)120
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MutationS110K, I111K
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GenBank IDCP001037.1 ACC81364.1
- Promoter Para
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Tag
/ Fusion Protein
- GB1-His6 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pBAD Forward
- 3′ sequencing primer pTrcHisrev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHBBAD113 was a gift from Hideo Iwai (Addgene plasmid # 121912 ; http://n2t.net/addgene:121912 ; RRID:Addgene_121912) -
For your References section:
The crystal structure of the naturally split gp41-1 intein guides the engineering of orthogonal split inteins from cis-splicing inteins. Beyer HM, Mikula KM, Li M, Wlodawer A, Iwai H. FEBS J. 2019 Oct 30. doi: 10.1111/febs.15113. 10.1111/febs.15113 PubMed 31665813
