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pCasAb-apr
(Plasmid #121998)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 121998 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Apramycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Selectable Marker: 100 μg/mL apramycin in both E.coli DH5alpha and Acinetobacter baumannii
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCasAb-apr was a gift from Quanjiang Ji (Addgene plasmid # 121998 ; http://n2t.net/addgene:121998 ; RRID:Addgene_121998)
  • For your References section:

    A Highly Efficient CRISPR-Cas9-Based Genome Engineering Platform in Acinetobacter baumannii to Understand the H2O2-Sensing Mechanism of OxyR. Wang Y, Wang Z, Chen Y, Hua X, Yu Y, Ji Q. Cell Chem Biol. 2019 Sep 17. pii: S2451-9456(19)30277-6. doi: 10.1016/j.chembiol.2019.09.003. 10.1016/j.chembiol.2019.09.003 PubMed 31548010