pCasAb-apr
(Plasmid #121998)

• Purpose: (Empty Backbone) Bacterial expression of Cas9 nuclease and RecAb recombination system in Acinetobacter baumannii
• Depositing Lab: Quanjiang Ji
• Publication: Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-9456(19)30277-6. doi: 10.1016/j.chembiol.2019.09.003.

Backbone

• Vector backbone: pMMB67EH
• Backbone size (bp): 16599
• Vector type: Bacterial Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Apramycin, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Selectable Marker: 100 μg/mL apramycin in both E.coli DH5alpha and Acinetobacter baumannii. Plasmid is prone to recombination, test 2-3 colonies.
• Copy number: Low Copy

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the plasmid is prone to recombination between the duplicate tac promoter-lac operator. Addgene recommends that you test 2-3 colonies via digest or sequencing to confirm that the full 16.6kb plasmid was selected.