pNIA-CEN-mVenus-FLAG-LANS1-Set2
(Plasmid #122003)

• Purpose: mVenus-FLAG-LANS-Set2: mVenus- and FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeast
• Depositing Lab: Brian Kuhlman
• Publication: Lerner et al Genome Res. 2020 Nov;30(11):1605-1617. doi: 10.1101/gr.264283.120. Epub 2020 Oct 5.

Backbone

• Vector backbone: pNIA-CEN
• Backbone size w/o insert (bp): 8039
• Total vector size (bp): 11531
• Vector type: Yeast Expression, Synthetic Biology
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mVenus-FLAG-LANS1-Set2
• Alt name: mVenus-FLAG-LANS-Set2
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 3492
• Mutation: Set2 point mutations K538G, K539S, R549G, K550S, K551G
• Entrez Gene: SET2 (a.k.a. YJL168C, EZL1, KMT3)
• Promoter: ADH1
• Tag / Fusion Protein:
  • mVenus-FLAG (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GTCATTGTTCTCGTTCCCTTTCTTCCTTG
• 3′ sequencing primer: GGGACCTAGACTTCAGGTTGTCTAACTCC

Resource Information

• Supplemental Documents:
  • pNIA-CEN-mVenus-FLAG-LANS1-Set2.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: the Addgene ID for this plasmid is incorrectly listed in the publication as 122002. Addgene plasmid 122003 is mVenus-Flag-LANS-Set2 and Addgene plasmid 122002 is Flag-LANS-Set2.