Addgene - pLV-tTRKRAB Plasmid Data

Gene/insert name:
TetR-KRAB fusion
Vector backbone:
pLV-tTRKRAB
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi, Cre/Lox
Backbone size w/o insert:
10713
5' sequencing primer:
See map List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Didier Trono
Terms and Licenses:
MTA
Tet Systems Limited Use Label License

Comments:

Older, double-copy vector system for conditional expression of siRNA. Has been replaced by the all-in-one vector system from Trono/Aebischer lab (e.g. see Addgene plasmid 11643).

For conditional expression of siRNA, the target cells need to be cotransduced with both pLVTH/pLVTHM and pLV-tTRKRAB/pLV-tTRKRAB-Red to achieve conditional expression of siRNA.

Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Conditional suppression of cellular genes: lentivirus vector-mediated drug-inducible RNA interference. Wiznerowicz et al (J Virol. 2003 Aug . 77(16):8957-61. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12249" in your Materials and Methods section.