Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pcDNA3.1-mPCSK3 H194L
(Plasmid #122689)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 122689 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 7809
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2381
  • Mutation
    Changed Histidine 194 to Leucine
  • GenBank ID
    NM_011046.3
  • Entrez Gene
    Furin (a.k.a. 9130404I01Rik, Fur, PACE, Pcsk3, SPC1)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-mPCSK3 H194L was a gift from Liming Pei (Addgene plasmid # 122689 ; http://n2t.net/addgene:122689 ; RRID:Addgene_122689)
  • For your References section:

    Growth Differentiation Factor 15 Maturation Requires Proteolytic Cleavage by PCSK3, -5, and -6. Li JJ, Liu J, Lupino K, Liu X, Zhang L, Pei L. Mol Cell Biol. 2018 Oct 15;38(21). pii: MCB.00249-18. doi: 10.1128/MCB.00249-18. Print 2018 Nov 1. 10.1128/MCB.00249-18 PubMed 30104250