pAC10094_pLX-DEST-IRES-TagBFP2
(Plasmid
#122814)
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PurposePlasmid 94:Lentiviral Gateway desination vector encoding TagBFP2
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 122814 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLX302 (Addgene #25896)
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Modifications to backbonePuromycin removed
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Vector typeMammalian Expression, Lentiviral ; Gateway Destination
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Selectable markersTagBFP2
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTagBFP2
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer TCCTCGGTCTCGATTCTACG
- 3′ sequencing primer agcagcgtatccacatagcg (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/452979v2 for BioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAC10094_pLX-DEST-IRES-TagBFP2 was a gift from Albert Cheng (Addgene plasmid # 122814) -
For your References section:
Split Selectable Markers. Jillette N, Du M, Cheng AW. bioRxiv 452979 10.1101/452979
