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(Plasmid #122871)


Item Catalog # Description Quantity Price (USD)
Plasmid 122871 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    human CCDC11
  • Alt name
  • Species
    H. sapiens (human)
  • Entrez Gene
    CFAP53 (a.k.a. CCDC11, HTX6)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer AATACGACTCACTATAG
  • 3′ sequencing primer M13R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

A human CCDC11 cDNA, kindly provided by Dr. Moe Mahjoub at Washington University, was subcloned into the V8 BB.GFPLT CS2+ vector (Addgene plasmid #17099).

The insert can be sequenced using the following primers: forward (GFP primer), ACCACATGGTCCTTCTTCAG;
reverse (T7 primer), TTATGCTGAGTGATATC.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-hCCDC11 was a gift from FENG-QIAN Li & Ken-Ichi Takemaru (Addgene plasmid # 122871 ; ; RRID:Addgene_122871)