Addgene - pBluescript SK +/- mTRF1 Plasmid Data

Plasmid 12303: pBluescript SK +/- mTRF1

Gene/insert name:
mTRF1
Alt name:
mouse TTAGGG repeat binding factor 1
Alt name:
840
Insert size:
1700
Species:
M. musculus (mouse)
GenBank ID:
u65586
Vector backbone:
pBluescript SK +/-
(Search Vector Database)
Backbone manufacturer:
Stratagene
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
2900
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
T3 List of Sequencing Primers
3' sequencing primer:
T7
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Titia de Lange
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Comparison of the human and mouse genes encoding the telomeric protein, TRF1: chromosomal localization, expression and conserved protein domains. Broccoli et al (Hum Mol Genet. 1997 Jan . 6(1):69-76. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12303" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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12306: pBluescript SK - hTRF1