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6972 pPROEX-HTb-SSA1
(Plasmid #1231)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 1231 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pPROEX-HTb
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6688
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ssa1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    SSA1 (a.k.a. YAL005C, YG100)
  • Tag / Fusion Protein
    • his tag (tev cleavable) (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ehe1 (not destroyed)
  • 3′ cloning site sac1 (not destroyed)
  • 5′ sequencing primer M13/pUC Reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Anil Cashikar

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    6972 pPROEX-HTb-SSA1 was a gift from Susan Lindquist (Addgene plasmid # 1231 ; http://n2t.net/addgene:1231 ; RRID:Addgene_1231)
  • For your References section:

    Hsp104 catalyzes formation and elimination of self-replicating Sup35 prion conformers. Shorter J, Lindquist S. Science 2004 Jun 18;304(5678):1793-7. Epub 2004 May 20. 10.1126/science.1098007 PubMed 15155912