Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

pLPhygSaCas9
(Plasmid #123262)

Print

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 123262 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSP72
  • Total vector size (bp) 8995
  • Vector type
    CRISPR ; Leishmania
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Staphylococcus aureus Cas9
  • Alt name
    SaCas9
  • Entrez Gene
    NEWENTRY

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Feng Zhang Addgene plasmid pX601

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLPhygSaCas9 was a gift from Greg Matlashewski (Addgene plasmid # 123262 ; http://n2t.net/addgene:123262 ; RRID:Addgene_123262)

  • For your References section:

    Single-Strand Annealing Plays a Major Role in Double-Strand DNA Break Repair following CRISPR-Cas9 Cleavage in Leishmania. Zhang WW, Matlashewski G. mSphere. 2019 Aug 21;4(4). pii: 4/4/e00408-19. doi: 10.1128/mSphere.00408-19. 10.1128/mSphere.00408-19 PubMed 31434745
Related items: