Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12393||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepet29 b(+)
- Backbone size w/o insert (bp) 5371
Vector typeBacterial Expression
Growth in Bacteria
Growth instructionsPlasmids are provided in DH5 alpha, but for protein expression, BL21(DE3)pLysS is recommended.
Copy numberHigh Copy
Gene/Insert nameTop7 V48V
Insert Size (bp)321
/ Fusion Protein
- 6xHis (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
A synthetic gene with the Top7 sequence under the control of the T7 promoter, a C-terminal 6X His tag, and codon usage optimized for Escherichia coli was obtained from BlueHeron Biotechnologies. The gene was then subcloned into the pet29 b(+) vector from Novagen.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Top7 V48V was a gift from David Baker (Addgene plasmid # 12393 ; http://n2t.net/addgene:12393 ; RRID:Addgene_12393)
For your References section:Design of a novel globular protein fold with atomic-level accuracy. Kuhlman B, Dantas G, Ireton GC, Varani G, Stoddard BL, Baker D. Science. 2003 Nov 21. 302(5649):1364-8. 10.1126/science.1089427 PubMed 14631033