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PL1
(Plasmid #12394)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12394 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Plasmids are provided in DH5 alpha, but for protein expression, BL21(DE3)pLysS is recommended.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PL1
  • Alt name
    Protein L
  • Insert Size (bp)
    288
  • Mutation
    RosettaDesign was used to design sequences. Alignment to wild-type can be seen in the article.
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A synthetic gene which placed the sequence under the control of the T7 promoter, a C-terminal 6X His tag, and codon usage optimized for Escherichia coli was obtained from BlueHeron Biotechnologies. The gene was then subcloned into the pet29 b(+) vector from Novagen.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PL1 was a gift from David Baker (Addgene plasmid # 12394 ; http://n2t.net/addgene:12394 ; RRID:Addgene_12394)
  • For your References section:

    A large scale test of computational protein design: folding and stability of nine completely redesigned globular proteins. Dantas G, Kuhlman B, Callender D, Wong M, Baker D. J Mol Biol. 2003 Sep 12. 332(2):449-60. 10.1016/S0022-2836(03)00888-X PubMed 12948494