Top7 Y17L K39E K40E V48V K55E
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12400||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepet29 b(+)
- Backbone size w/o insert (bp) 5371
Vector typeBacterial Expression
Growth in Bacteria
Growth instructionsPlasmids are provided in DH5 alpha, but for protein expression, BL21(DE3)pLysS is recommended.
Copy numberHigh Copy
Gene/Insert nameTop7 Y19L K39E K40E V48V K55E
Insert Size (bp)321
MutationY19L K39E K40E V48V K55E
/ Fusion Protein
- 6xHis (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Top7 Y17L K39E K40E V48V K55E was a gift from David Baker (Addgene plasmid # 12400 ; http://n2t.net/addgene:12400 ; RRID:Addgene_12400)