Addgene - pFlagCMV2-14-3-3sigma Plasmid Data

Plasmid 12453: pFlagCMV2-14-3-3sigma

Gene/insert name:
14-3-3 sigma
Species:
H. sapiens (human)
Entrez Gene:
SFN (YWHAS)
Fusion protein or tag:
FLAG
Terminal:
N terminal on backbone
Vector backbone:
pFlag-CMV2
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4679
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
pCEP fwd List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Dong-Er Zhang
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The interferon-inducible ubiquitin-protein isopeptide ligase (E3) EFP also functions as an ISG15 E3 ligase. Zou et al (J Biol Chem. 2006 Feb 17. 281(7):3989-94. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12453" in your Materials and Methods section.