Purpose(Empty Backbone) Destination cloning vector for tRNA-flanked sgRNA. A BpiI placeholder is present between a minimal ADE promoter and SV40 polyA sequence.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||124547||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneP1-EYFP-pA (Construct 5) (Addgene: 55197)
Vector typeMammalian Expression
Growth in Bacteria
Destination cloning vector for custom tRNA-flanked gRNA combinations. A BpiI placeholder is included after the minimal ADE promoter to allow GoldenGate assembly. Enhancer elements can be exchanged using the NheI site, and promoter/enhancer region can be changed using NcoI NheI double digestion.
Please visit https://www.biorxiv.org/content/10.1101/342485v1 for BioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:8xCTS1_BpiI-cloning-site_SV40polyA was a gift from Tudor Fulga (Addgene plasmid # 124547 ; http://n2t.net/addgene:124547 ; RRID:Addgene_124547)