PurposeFive prime entry vector containing 349bp cldn15la promoter from zebrafish. Drives expression in intestinal epithelial cells.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||125026||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonep5E-Fse-Asc 381
- Backbone size w/o insert (bp) 2663
- Total vector size (bp) 3012
Growth in Bacteria
Gene/Insert nameClaudin 15-like a
SpeciesD. rerio (zebrafish)
Insert Size (bp)349
Entrez Genecldn15la (a.k.a. cldn10, cldn10l, cldn10l1a)
- Cloning method Restriction Enzyme
- 5′ cloning site FseI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p5E-0.349cldn15la was a gift from John Rawls (Addgene plasmid # 125026 ; http://n2t.net/addgene:125026 ; RRID:Addgene_125026)
For your References section:Intestinal Serum amyloid A suppresses systemic neutrophil activation and bactericidal activity in response to microbiota colonization. Murdoch CC, Espenschied ST, Matty MA, Mueller O, Tobin DM, Rawls JF. PLoS Pathog. 2019 Mar 7;15(3):e1007381. doi: 10.1371/journal.ppat.1007381. eCollection 2019 Mar. 10.1371/journal.ppat.1007381 PubMed 30845179