PurposeStorage plasmid for K.marxianus left homology arm; type 1 part
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||125033||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerDueber lab, UC Berkeley
- Backbone size w/o insert (bp) 1667
Vector typeYeast Expression, Synthetic Biology
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameLeft homology arm for targeting integration to chromosome IV:240042..241741
Insert Size (bp)880
- Cloning method Gibson Cloning
- 5′ sequencing primer tttgctggccttttgctc
- 3′ sequencing primer catctggatttgttcagaacg (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Meant for the construction of integrative vectors (single- or multi-gene). Contains the appropriate YTK-compatible Golden Gate overhangs for a type 1 part. The necessary internal BsmBI sites to use this as a connector for multi-gene assembly have been added by PCR.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pKmK.I4L was a gift from John Morrissey (Addgene plasmid # 125033 ; http://n2t.net/addgene:125033 ; RRID:Addgene_125033)
For your References section:Biological Parts for Kluyveromyces marxianus Synthetic Biology. Arun S. Rajkumar, Javier A. Varela, Hannes Juergens, Jean-Marc G. Dara2 and John P. Morrissey. Front. Bioeng. Biotechnol., 07 May 2019 10.3389/fbioe.2019.00097