pAGW-dpse-GAL4-DBD
(Plasmid #125153)

• Purpose: Gateway-compatible vector to express a fusion protein containing the GAL4-DBD (N-terminally) followed by the protein of interest under the control of a D.pseudoobscura enhancer and CP
• Depositing Lab: Alexander Stark
• Publication: Haberle et al Nature. 2019 Jun;570(7759):122-126. doi: 10.1038/s41586-019-1210-7. Epub 2019 May 15.

Backbone

• Vector backbone: Unknown
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 2962
• Vector type: Insect Expression ; Gateway fly expression vector

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: D. pseudoobscura peak #102 enhancer and D. pseudoobscura CG13116 CP
• Insert Size (bp): 1213

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • pAGW-dpse-GAL4-DBD.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry